Journal: Cell chemical biology

Article Title: Antibiotic target discovery by integrated phenotypic and activity-based profiling of electrophilic fragments.

doi: 10.1016/j.chembiol.2025.02.001

Figure Lengend Snippet: Figure 1. Bacterial growth inhibition screen using an electrophilic cysteine-reactive fragment library at 25 mM Relative bacterial growth was determined by normalizing the OD600 value to the DMSO control. (A) Growth inhibition screen in S. aureus (MSSA476). (B) Growth inhibition screen in V. cholerae (SAD30). Hits (relative growth %10%) are labeled in red.

Article Snippet: E. coli BAA-2340, A. baumannii BAA-1790, P. aeruginosa BAA-2110, K. pneumoniae 700603, K. pneumoniae 13883, S. aureus MSSA476, S. aureus 43300, E. cloacae 13047, E. coli K12 were purchased directly from ATCC.

Techniques: Inhibition, Control, Labeling

Journal: Cell chemical biology

Article Title: Antibiotic target discovery by integrated phenotypic and activity-based profiling of electrophilic fragments.

doi: 10.1016/j.chembiol.2025.02.001

Figure Lengend Snippet: Figure 2. Antibacterial activity of and resistance development to 10-F05 (A) Time-dependent killing of S. flexneri M90T by 10-F05 (n = 2, mean ± SD). (B) Resistance development of S. flexneri M90T to 10-F05 and kanamycin during daily serial passaging with sub-MIC concentrations. (C) Resistance development of S. aureus MSSA476 to 10-F05 and methicillin during daily serial passaging with sub-MIC concentrations. (D) MIC fold change of the 10-F05-resistant S. aureus MSSA476 toward other classes of antibiotics. MIC values were reported in supplementary data.

Article Snippet: E. coli BAA-2340, A. baumannii BAA-1790, P. aeruginosa BAA-2110, K. pneumoniae 700603, K. pneumoniae 13883, S. aureus MSSA476, S. aureus 43300, E. cloacae 13047, E. coli K12 were purchased directly from ATCC.

Techniques: Activity Assay, Passaging

Journal: Cell chemical biology

Article Title: Antibiotic target discovery by integrated phenotypic and activity-based profiling of electrophilic fragments.

doi: 10.1016/j.chembiol.2025.02.001

Figure Lengend Snippet: Figure 4. Chemogenetic validations suggested that FabH is the major target of 10-F05-mediated growth inhibition (A) Structural-based sequence alignment of FabH proteins from different bacteria species. BS, B. subtilis strain 168; SA, S. aureus MSSA476; EF, E. faecium; PA, P. aeruginosa ATCC 15692; EC, E. coli K12; SF, S. flexneri M90T; En, Enterobacter sp. (strain 638); KP, K. pneumoniae subsp. pneumoniae ATCC 700721; VC, V. cholerae serotype O1; AB, A. baumannii NCGM 237. The conserved cysteines are highlighted with a red box.

Article Snippet: E. coli BAA-2340, A. baumannii BAA-1790, P. aeruginosa BAA-2110, K. pneumoniae 700603, K. pneumoniae 13883, S. aureus MSSA476, S. aureus 43300, E. cloacae 13047, E. coli K12 were purchased directly from ATCC.

Techniques: Inhibition, Sequencing, Bacteria

Journal: Antibiotics

Article Title: The Therapeutic Potential of Agarwood as an Antimicrobial and Anti-Inflammatory Agent: A Scoping Review

doi: 10.3390/antibiotics13111074

Figure Lengend Snippet: Tabulated summary of articles included in this review.

Article Snippet: 12 , [ ] , To determine the antibacterial activity of agarwood bouya ( Aquilaria agallocha ) oil nanoemulsion against multidrug-resistant bacteria (MDR) and antibiotic-nonresistant bacteria. , Disk diffusion method. , The 20% agarwood bouya oil nanoemulsion could only suppress E. coli ATCC 43300, which produces ESBLs, by 3.3 mm, while S. aureus ATCC 25923 and K. pneumoniae ATCC 8724 have inhibition zones of 2.6 mm and 3.3 mm, respectively. The non-resistant E. coli ATCC 25922 bacteria showed active inhibition, with an inhibition zone of 13.3 mm. , The oil nanoemulsion’s ability to inhibit multidrug-resistant bacteria (MDR) will increase with its concentration..

Techniques: Bacteria, In Vitro, Activity Assay, Diffusion-based Assay, Thin Layer Chromatography, Infection, Concentration Assay, Produced, Antioxidant Activity Assay, Incubation, In Silico, Mutagenesis, Migration, Inhibition, Virus, Plasmid Preparation, Control, Extraction, Analogues, Isolation, Protein-Protein interactions, In Vivo, Cell Culture, Viability Assay, Activation Assay, Generated, Circular Dichroism, Griess Assay, DPPH Assay

Journal: Foods

Article Title: Lactic Acid Bacteria as the Green and Safe Food Preservatives: Their Mechanisms, Applications and Prospects

doi: 10.3390/foods15020241

Figure Lengend Snippet: The inhibitory mechanism of LAB on foodborne microorganisms.

Article Snippet: Similarly, Choi et al. demonstrated that LAB, including Lacticaseibacillus curvatus KCCM 43119, Leuconostoc mesenteroides KCCM 43060, Weissella cibaria KCTC 3746, and Weissella koreensis KCCM 41517, could almost completely prevent the growth of four foodborne pathogens ( Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, Staphylococcus aureus KCCM 11335, and Escherichia coli O157:H7 ATCC 35150), increasing the inhibition ratio from 93% to 100% [ ].

Techniques: